7061 spore protein extracts Search Results


7061 spores  (ATCC)
95
ATCC 7061 spores
SDS-PAGE analysis <t>of</t> <t>proteins</t> extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
7061 Spores, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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small acid soluble spore protein b  (ATCC)
96
ATCC small acid soluble spore protein b
SDS-PAGE analysis <t>of</t> <t>proteins</t> extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
Small Acid Soluble Spore Protein B, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav vectors  (Vector Biolabs)
95
Vector Biolabs aav vectors
SDS-PAGE analysis <t>of</t> <t>proteins</t> extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
Aav Vectors, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 6  (Thermo Fisher)
86
Thermo Fisher il 6
Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, <t>TNF-α,</t> <t>IL-6</t> in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.
Il 6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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complement 1065 yp 002317098 1 1426 anoxybacillus  (ATCC)
93
ATCC complement 1065 yp 002317098 1 1426 anoxybacillus
Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, <t>TNF-α,</t> <t>IL-6</t> in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.
Complement 1065 Yp 002317098 1 1426 Anoxybacillus, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc anti mouse il 6  (Thermo Fisher)
86
Thermo Fisher fitc anti mouse il 6
Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, <t>TNF-α,</t> <t>IL-6</t> in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.
Fitc Anti Mouse Il 6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti–il-6 (low endotoxin, azide free; mp5-20f3; 16-7061)  (Thermo Fisher)
90
Thermo Fisher anti–il-6 (low endotoxin, azide free; mp5-20f3; 16-7061)
Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, <t>TNF-α,</t> <t>IL-6</t> in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.
Anti–Il 6 (Low Endotoxin, Azide Free; Mp5 20f3; 16 7061), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-mouse il-6 cat# 16-7061 clone mp5-20f3  (Thermo Fisher)
90
Thermo Fisher anti-mouse il-6 cat# 16-7061 clone mp5-20f3
Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, <t>TNF-α,</t> <t>IL-6</t> in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.
Anti Mouse Il 6 Cat# 16 7061 Clone Mp5 20f3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il-6 #11-7061-82  (Thermo Fisher)
90
Thermo Fisher il-6 #11-7061-82
Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, <t>TNF-α,</t> <t>IL-6</t> in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.
Il 6 #11 7061 82, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa mouse il-6 250x detection antibody  (Thermo Fisher)
90
Thermo Fisher elisa mouse il-6 250x detection antibody
Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, <t>TNF-α,</t> <t>IL-6</t> in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.
Elisa Mouse Il 6 250x Detection Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-mouse il-6 capture antibody (1:250; 14-7061-68)  (Thermo Fisher)
90
Thermo Fisher anti-mouse il-6 capture antibody (1:250; 14-7061-68)
Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, <t>TNF-α,</t> <t>IL-6</t> in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.
Anti Mouse Il 6 Capture Antibody (1:250; 14 7061 68), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits for tnf-α #88-7324-86  (Thermo Fisher)
90
Thermo Fisher elisa kits for tnf-α #88-7324-86
Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, <t>TNF-α,</t> <t>IL-6</t> in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.
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Image Search Results


SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

Journal: Applied and Environmental Microbiology

Article Title: Protection of Bacillus pumilus Spores by Catalases

doi: 10.1128/AEM.01211-12

Figure Lengend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

Article Snippet: We performed spore decoating by using detergent in the presence of salt and a reducing agent under alkaline conditions to extract the coat proteins of SAFR-032 and ATCC 7061 spores.

Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy

SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

Journal: Applied and Environmental Microbiology

Article Title: Protection of Bacillus pumilus Spores by Catalases

doi: 10.1128/AEM.01211-12

Figure Lengend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

Article Snippet: We performed spore decoating by using detergent in the presence of salt and a reducing agent under alkaline conditions to extract the coat proteins of SAFR-032 and ATCC 7061 spores.

Techniques: SDS Page

Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, TNF-α, IL-6 in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Depletion of microglia exacerbates postischemic inflammation and brain injury

doi: 10.1177/0271678X17694185

Figure Lengend Snippet: Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)–(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1α, IL-1β, iNOS, CCL2, TNF-α, IL-6 in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.

Article Snippet: The following antibodies were used: CD3 (145-2C11, 553066, BD Biosciences, San Jose, CA), CD4 (RM4-5, 552775, BD Biosciences, San Jose, CA), CD8 (53-6.7, 557654, BD Biosciences, San Jose, CA), NK1.1 (PK136, 551114, BD Biosciences, San Jose, CA), CD45 (30-F11, 12-0451-83, eBioscience, San Diego, CA), CD11b (M1/70, 25-0112-82, eBioscience, San Diego, CA), F4/80 (BM8, 123119, Biolegend, San Diego, CA), Ly6G (1A8, 127614, Biolegend, San Diego, CA), B220 (RA3-6B2, 553093, BD Biosciences, San Jose, CA), IL-1α (559810, BD Biosciences, San Jose, CA), IL-1β (NJTEN3, 12-7114-82, eBioscience, San Diego, CA), IL-6 (MP5-20F3, 12-7061-81, eBioscience, San Diego, CA), MCP-1 (2H5, 505903, Biolegend, San Diego, CA), TNFα (MP6-XT22, 557719, BD Biosciences, San Jose, CA), GFAP (GA5, 50-9892-82, eBioscience, San Diego, CA), iNOS (CXNFT, 53-5920-80, eBioscience, San Diego, CA).

Techniques: Flow Cytometry, Expressing

Microglia restricted ischemia-induced astrocyte response and neural injury. Primary cortical neurons were subjected to 5 min OGD. Immediately after OGD, culture media was replaced with regular neurobasal medium mixed with equal volume of glia-conditioned medium for 24 h. Glia-conditioned medium was obtained from cultured microglia, astrocytes or mixed microglia and astrocytes (microglia/astrocyte) at 24 h after 30 min OGD. (a) Representative images show staining of beta III Tubulin (green), DAPI (blue) and TUNEL (red) in primary cortical neurons exposed to OGD and glia-conditioned medium as indicated. Primary cortical neurons without exposure to OGD or glia-conditioned medium were used as controls. Scale bars: 20 µm. (b) Quantification of TUNEL + cells after exposure to indicated treatment. CM: conditional medium. (c) Bar graphs show the level of released LDH from primary cortical neurons after exposure to indicated treatment. LDH release was expressed by normalizing the released LDH in the medium to the total LDH (released + intracellular). CM: conditional medium. (d) Primary astrocytes were cultured separately or together with microglia (microglia/astrocyte). After 30 min OGD and subsequent 24 h recovery, intracellular staining of GFAP and indicated cytokines was performed and analyzed by flow cytometry. Bar graphs show the percentage of GFAP + cells expressing IL-1α, IL-1β, iNOS, CCL2, TNF-α and IL-6. Error bars represent SD.; * P < 0.05; ** P < 0.01.

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Depletion of microglia exacerbates postischemic inflammation and brain injury

doi: 10.1177/0271678X17694185

Figure Lengend Snippet: Microglia restricted ischemia-induced astrocyte response and neural injury. Primary cortical neurons were subjected to 5 min OGD. Immediately after OGD, culture media was replaced with regular neurobasal medium mixed with equal volume of glia-conditioned medium for 24 h. Glia-conditioned medium was obtained from cultured microglia, astrocytes or mixed microglia and astrocytes (microglia/astrocyte) at 24 h after 30 min OGD. (a) Representative images show staining of beta III Tubulin (green), DAPI (blue) and TUNEL (red) in primary cortical neurons exposed to OGD and glia-conditioned medium as indicated. Primary cortical neurons without exposure to OGD or glia-conditioned medium were used as controls. Scale bars: 20 µm. (b) Quantification of TUNEL + cells after exposure to indicated treatment. CM: conditional medium. (c) Bar graphs show the level of released LDH from primary cortical neurons after exposure to indicated treatment. LDH release was expressed by normalizing the released LDH in the medium to the total LDH (released + intracellular). CM: conditional medium. (d) Primary astrocytes were cultured separately or together with microglia (microglia/astrocyte). After 30 min OGD and subsequent 24 h recovery, intracellular staining of GFAP and indicated cytokines was performed and analyzed by flow cytometry. Bar graphs show the percentage of GFAP + cells expressing IL-1α, IL-1β, iNOS, CCL2, TNF-α and IL-6. Error bars represent SD.; * P < 0.05; ** P < 0.01.

Article Snippet: The following antibodies were used: CD3 (145-2C11, 553066, BD Biosciences, San Jose, CA), CD4 (RM4-5, 552775, BD Biosciences, San Jose, CA), CD8 (53-6.7, 557654, BD Biosciences, San Jose, CA), NK1.1 (PK136, 551114, BD Biosciences, San Jose, CA), CD45 (30-F11, 12-0451-83, eBioscience, San Diego, CA), CD11b (M1/70, 25-0112-82, eBioscience, San Diego, CA), F4/80 (BM8, 123119, Biolegend, San Diego, CA), Ly6G (1A8, 127614, Biolegend, San Diego, CA), B220 (RA3-6B2, 553093, BD Biosciences, San Jose, CA), IL-1α (559810, BD Biosciences, San Jose, CA), IL-1β (NJTEN3, 12-7114-82, eBioscience, San Diego, CA), IL-6 (MP5-20F3, 12-7061-81, eBioscience, San Diego, CA), MCP-1 (2H5, 505903, Biolegend, San Diego, CA), TNFα (MP6-XT22, 557719, BD Biosciences, San Jose, CA), GFAP (GA5, 50-9892-82, eBioscience, San Diego, CA), iNOS (CXNFT, 53-5920-80, eBioscience, San Diego, CA).

Techniques: Cell Culture, Staining, TUNEL Assay, Flow Cytometry, Expressing